Composite

Part:BBa_K2477008:Design

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)


GFP based fusaric acid biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1527


Design Notes

We had to consider that the inducing substances actually increase repression rather than decreasing it like typical systems.


Source

Registry parts. PhlF comes from Pseudomonas fluorescens.

References